analyzeLiterature
If you have access to the bustalab server, you can run the command analyzeLiterature() in an R chunk and connect to a shiny app that stores the Busta Lab’s literature database. Here are some example questions that you can ask of our literature database:
Simple question: What are wax blooms?
Medium-Complexity question: How are ABC transporters involved in the movement of cuticle-related compounds?
High-Complexity question: Can you describe what is known about the transcriptional regulation of lipid transfer proteins in plants?
Can you explain - Conceptual: I don’t understand the following passage, can you summarize it in simple terms?
“In earlier studies, different other compounds including β-amyrin were overproduced through using strong constitutive promoters [12], enhancers [13] and transcription factors [14]. The β-amyrin is the triterpenoids belongs to oleanane group [15], which harbors anti-hyperglycemic, anti-inflammatory, hypolipidemic effects along with several other pharmacological activities [16,17]. The β-amyrin synthase (βAS) is responsible to synthesize the β-amyrin from 2,3-oxidosqualene[18]. This 2,3-oxidosqualene is synthesized from squalene through squalene epoxidase (SQE), encoded by the ERG1 gene in S. cerevisiae [19]. Squalene is synthesized from farnesyl-pyrophosphate through the action of squalene synthase (SQS) (ERG9 gene); and this farnesyl-pyrophosphate (FPP) is synthesized by farnesyl diphosphate synthase (FPPS) (ERG20 gene), from isopentenyl pyrophosphate (IPP), a precursor supplied by mevalonate (MVA) pathway (Fig. 1). The 3-Hydroxy-3-Methyl glutaryl-CoA reductase (HMG1) [20,21] and squalene monooxygenase or SQE [22] are the rate-limiting enzymes of the terpenoid pathway in yeast. For the biosynthesis of triterpenoids, SQS and SQE are important enzymes [23] and were previously overexpressed for the overproduction of triterpenoids [14,24].”
- Can you explain - Method/technique: Can you summarize the GAL4/RUBY assay in simple terms?